Intracellular Localization of Some Key Enzymes of Crassulacean Acid Metabolism in Sedum praealtum
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منابع مشابه
Intracellular Localization of Some Key Enzymes of Crassulacean Acid Metabolism in Sedum praealtum.
The intracellular locations of six key enzymes of Crassulacean acid metabolism were determined using enzymically isolated mesophyll protoplasts of Sedum praealtum D.C. Data from isopycnic sucrose density gradient centrifugation established the chloroplastic location of pyruvate Pi dikinase, the mitochondrial location of NAD-linked malic enzyme, and exclusively nonparticulate (not associated wit...
متن کاملIsolation and Oxidative Properties of Intact Mitochondria from the Leaves of Sedum praealtum: A Crassulacean Acid Metabolism Plant.
A procedure is described for preparing intact mitochondria from leaves of Sedum praealtum D.C., a plant showing Crassulacean acid metabolism. These mitochondria oxidized malate, pyruvate, alpha-ketoglutarate, succinate, NADH, NADPH, and isocitrate with good respiratory control and ADP/O ratios better than those observed in mitochondria from other photosynthetic tissues.Malate oxidation was very...
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Enzymes involved in malic acid production via a pathway with 2 carboxylation reactions and in malic acid conversion via total oxidation have been demonstrated in mitochondria of Bryophyllum tubiflorum Harv. Activation of the mitochondria by Tween 40 was necessary to reveal part of the enzyme activities. The temperature behavior of the enzymes has been investigated, revealing optimal activity of...
متن کاملLight modulation of the activity of carbon metabolism enzymes in the crassulacean Acid metabolism plant kalanchoë.
When intact Kalanchoë plants are illuminated NADP-linked malic dehydrogenase and three enzymes of the reductive pentose phosphate pathway, ribulose-5-phosphate kinase, NADP-linked glyceraldehyde-3-phosphate dehydrogenase, and sedoheptulose-1,7-diphosphate phosphatase, are activated. In crude extracts these enzymes are activated by dithiothreitol treatment. Light or dithiothreitol treatment does...
متن کاملCrassulacean Acid Metabolism
Crassulacean acid metabolism (CAM) is a CO2 acquisition, CO2 transient storage and CO2 concentrating mechanism based on organic acid synthesis. CO2 acquisition occurs in the dark. CO2 is fixed and stored in the form of organic acids. Remobilization of CO2 from the organic acids occurs in the light and leads to massive CO2 concentration inside photosynthesizing plant organs (green leaves or stem...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1979
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.63.4.738